Fig. 5
From: Lipid nanoparticle-based mRNA delivery systems for cancer immunotherapy
(Reproduced from a previous report [6] with permission from American Chemical Society)
CAR-encoded mRNA-LNP delivery system. a Schematic representation of T-cell targeting using CAR mRNA-loaded LNPs. b Fabrication of LNPs with various components using microfluidic technology. c Expression rate of CAR in primary T cells analyzed by flow cytometry, with both purified LNP and electroporation (EP) groups showing an increase in CAR expression on T cells. d Cell viability assessment, highlighting that the EP group exhibited the lowest cell viability among primary T cells.