From: Designer nanoparticle: nanobiotechnology tool for cell biology
Subcellular Organelles | Isolation techniques |
---|---|
Cell wall | Gradient centrifugation |
Chloroplast | Gradient centrifugation antibody based pull-down assay, SPMNPS: Tag-anti-tag; Antibody conjugated; Biotin-streptavidin |
Cilia and flagella | Gradient centrifugation Antibody based pull-down assay; Tag-anti-tag; Antibody conjugated; Biotin-streptavidin |
Cytoplasm | Gradient centrifugation antibody based pull-down assay; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin tagged SPMNPs |
Cytoskeleton | Gradient centrifugation antibody based pull-down assay; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin SPMNPs |
Early endosomes | Gradient centrifugation SPMNP isolation assay antibody based pull-down assay; Biotin-streptavidin tagged SPMNPs; antibody-SPMNPs; Negatively charged lipid-SPMNPs |
Endoplasmic reticulum (ER)—rough or smooth | Gradient centrifugation antibody based pull-down assay; Tag-anti-tag; Antibody conjugated; Biotin-streptavidin tagged SPMNPs |
Golgi apparatus | Gradient centrifugation antibody based pull-down assay; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin tagged SPMNPs |
Late endosomes | Gradient centrifugation SPMNP isolation assay; Biotin-streptavidin; antibody-SPMNPs; Negatively charged SPMNPs |
Multi-vesicular bodies | Gradient centrifugation SPMNP isolation assay; antibody based pull-down assay; Biotin-streptavidin; antibody-SPMNPs |
Nucleus | Gradient centrifugation antibody based pull-down assay; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin |
Peroxisomes | Gradient centrifugation antibody based pull-down assay; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin tagged SPMNPs |
Phagosomes | Gradient centrifugation SPMNP isolation assay Antibody based pull-down assay; Biotin-streptavidin; antibody SPMNPs |
Lysosomes | Gradient centrifugation SPMNP isolation assay Antibody based pull-down assay; Biotin-streptavidin tagged SPMNPs; antibody |
Plasma Membrane or Cell Membrane | Gradient centrifugation; Cationic silica beads; SPMNP isolation; antibody conjugated; Biotin-Streptavidin; lectin–SPMNPs |
Ribosomes | Gradient centrifugation; pull -down assay; anti-S10/anti–EF-Tu-SPMNPs; Biotin-streptavidin; antibody- SPMNPs |
Lipid rafts | Gradient centrifugation antibody based pull-down assay; Tag-antitagged-SPMNPs; Biotin-streptavidin; protein conjugated magnetic isolation |
Secretory granules or vesicles | Gradient centrifugation antibody based pull-down assay; Tag-antitagged SPMNPs; Biotin-streptavidin-SPMNPs |
Synaptosomes | Gradient centrifugation antibody based pull-down assay; Tag-anti-tag SPMNPs; Antibody conjugated; Biotin-streptavidin SPMNPs |
Vacuoles | Gradient centrifugation; antibody based pull-down assay; antibody conjugated magnetic nanoparticles |
Mitochondria | Gradient centrifugation; pull-down assay; antibody conjugated SPMNPs; Tag-anti-tagged; Antibody conjugated; Biotin-streptavidin; Anti-TOM22 antibody tagged SPMNPs |