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TableĀ 3 Comparison of existing technologies for endosomes and lysosome isolation

From: Designer nanoparticle: nanobiotechnology tool for cell biology

Isolation methodologies Advantages Disadvantages
Density gradient centrifugation; Conventional method that can be used to isolate endosomes and lysosomes along other subcellular compartments
Effective method to isolate lysosomes from tissue or in vivo cell fractions
Simple procedure that can be performed using an ultracentrifuge
Low yield
Difficulties in separating endosomes from lysosome vesicles
Difficulties in separating different endosomal and its associated vesicles
Antibody based pull-down assay Can be used to pull down proteins after post-fractionation
Can also be used to target selective endocytic uptakes
Can be used in combination with biotin-streptavidin assay
Limited applicability for certain endocytosis uptake
Limited yield and low purity
Cannot isolate vesicles under native conditions
SPMNPs based isolation; A novel strategy that is generic for any kind of cell systems with reasonable purity and yield
Method does not involve the use of detergent or antibody that affects native conditions
Method can also use targeted endosomal uptake pathway by using ligand tagged nanoparticle
Can be used to isolate protein under native conditions and all endosomal uptake
Technology has not be established for isolation of vesicles form tissue cells and in vivo experiments
Technology has not been established to isolate vesicles from cell suspension. Possibility exists to use this technology for cells in suspension culture