Fig. 9

Enhanced oligodendrocyte differentiation on PCL-GO nanofiber-nanomaterial hybrid scaffold. Immunocytochemistry image of NSCs after 6 days of culture on hybrid scaffolds, stained for the early oligodendrocyte marker Olig2 (a) and the mature oligodendrocyte marker MBP (b). Scale bars 20 µm. Quantitative comparison on various substrates of the percentage of cells expressing Olig2 (c) and MBP (d). Quantitative PCR analysis showing gene expression of early oligodendrocyte markers including CNP, PDGFR, Olig2 and Olig2 (e), and mature oligodendrocyte markers including PLP, MBP, MAG and MOG (f). The gene expression is relative to GAPDH, and normalized to the conventional PLL-coated glass control [70]