Fig. 9
From: Recent advances in M13 bacteriophage-based optical sensing applications
Reprinted with permission from Royal Society of Chemistry © 2016
a The structures of M13-β-CD, Ada-FITC, and Ada-RhB. b Schematic of the FRET based ratiometric fluorescent pH nanosensor. c Normalized absorption spectra of Ada-FITC (green solid) and Ada-RhB (red solid), normalized fluorescence spectra of Ada-FITC (green dot) and Ada-RhB (red dot), respectively. d Fluorescence spectra of the mixture of Ada-FITC and Ada-RhB (green) or M13-b-CD/Ada-FITC/Ada-(red) under 450 nm excitation. The inset shows the image of solution in the absence (green) and presence (yellow) of M13 phage. e pH dependence of M13-β-CD/Ada-FITC/Ada-RhB complex. f The peak emission ratio between Ada-FITC (515 nm) and Ada-RhB (580 nm) shows linear pH dependence [34].