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Fig. 2 | Nano Convergence

Fig. 2

From: Guiding osteogenesis of mesenchymal stem cells using carbon-based nanomaterials

Fig. 2

A The reprint of this figure from [21] is permitted by the Elsevier. B The reprint of this figure from [25] is permitted by MDPI C, D The re-print of this figure from [26] is permitted by Dove Medical Press

A Concentration of vinculin (a), talin (b), osteocalcin (c) and ICAM-1 (d) in osteoblast-like MG 63 cells on day 8 after seeding on the pure terpolymer of polytetrafluoroethylene, polyvinyldifluoride and polypropylene (Ter), terpolymer mixed with 4 wt% of single-wall carbon nanohorns (SWNH) or 4 wt% of high crystalline electric arc multi-wall carbon nanotubes (MWNT-A) and tissue culture polystyrene (TCPS). B Immunofluorescence staining of markers of DNA damage response: 53BP1 (green) and gamma-H2AX (red) in human osteoblast-like U-2 OS cells on micropatterned or continuous fresh and aged fullerene films after 7 days of cultivation. GS, microscopic glass coverslips, reference material; GS + NCS, positive control to DNA damage response, induced by 1 h incubation of U-2 OS cells in neocarzinostain (NCS; 700 ng/mL). Fullerol pre-treatment increased osteogenic potential of human aDscs. Cells were pretreated with fullerol (1.0 or 0.1 µM) for 7 days, followed by osteogenic induction for 14 days (n = 4). C Gene expression of osteogenic markers, Runx2, OCN, and ALP by real-time rT-Pcr at day 7, using 18 s as internal control. D Alizarin red staining at day 21. Letters a and b denote p = 0.05 versus BM and gP/DeX group, respectively.

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