Fig. 8
From: Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species
a Chromophore and their environment in TagRFP (Reproduced from [97] with the permission of Elsevier). The chromophore backbone for TagRFP is shown in orange. The hydrogen bonds are indicated with the green dashed lines, the atoms are colored by the atom type, and the water molecules are shown as the red spheres. b, c Detection of 1O2 photogenerated from TagRFP (Reproduced from [70] with the permission of John Wiley and Sons). b 1O2 phosphorescence photosensitized by TagRFP in a PBS:Glycerol:d-PBS mixture (1:1:20) upon irradiation at λex = 532 nm. The luminescence signal at λ = 1270 nm was fit with a triexponential function. c Time course of fluorescent spectra of optically matched solutions of SOSG (λex = 480 nm) and TagRFP under light irradiation at λex = 532 nm. The increased amount of 1O2 was detected by the increase in the SOSG band (λem = 527 nm) with irradiation at λex = 532 nm. The concomitant decrease in the TagRFP band (λem = 590 nm) indicated photobleaching and/or photoconversion