Fig. 9
From: Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species
a 3D structure of the H-bond network in the vicinity of the trans chromophore (shown in green) for the mKate_pH2.0 structure (left) and the cis chromophore in the mKate_pH7.0 structure (right). Mediating waters are shown by red spheres (Reproduced from [102] with the permission of American Society for Biochemistry and Molecular Biology). b, c Detection of ROS generated by mKate2 embedded in silk (i.e. RFP fluorescent silk produced from silkworm transgenesis) using turn-on/off fluorescent probes upon green light irradiation at λex = 532 nm [71]. Fluorescent emission signals of the probes were monitored from solutions containing mKate2 fluorescent silk. b O •‒2 mediated by Type I photosensitization reaction, captured by turn-on fluorescent signals of TEMPO-9-ac (λex = 365 nm). c 1O2 mediated by Type II photosensitization reaction, detected by reduction in the original ABDA fluorescent intensity (λex = 365 nm)