Table 1 NWA effectiveness compared with conventional ELISA method
From: Innovations in biomedical nanoengineering: nanowell array biosensor
Method | ELISA_fluorescence expression | Electrochemical reaction |
|---|---|---|
Basic principle | Indirect | Direct redox reaction |
Limit of detection | 0.2 × 10−2 U/L (μmol/L) | 0.13 × 10−4 U/L (μmol/L) |
Sample volume | 200 μL per test | 5 μL per test |
Analysis time | 1Â h | 3Â min |
Detection range | Narrow (10 ng–1 μg/mL) | Wide(10 pg–10 μg/mL) |