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Fig. 2 | Nano Convergence

Fig. 2

From: Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF–SIMS) imaging

Fig. 2

(Reproduced from ref. [83] with permission, © John Wiley & Sons, Ltd)

ToF–SIMS depth profiles of H3O+, C5H15NPO4+, C27H45+, and C27H45O+ peaks in corpus callosum in coronal section and ToF–SIMS images of cholesterol and sulfatide ion peaks of the rat brain tissue. The blue square indicates the position for depth profile acquisition. Depth profiles of H3O+ at m/z 19, C5H15NPO4+ at m/z 184, C27H45+ at m/z 369 and C27H45O+ at m/z 385 obtained at the corpus callosum prepared using a thaw-mounted rat brain on a stainless steel plate and freeze-drying, b tape-supported rat brain on a stainless steel plate and freeze-drying, c thaw-mounted rat brain on a stainless steel plate and room temperature-drying, d thaw-mounted rat brain on a stainless steel plate and frozen-hydrate analysis, and e tape-supported rat brain and frozen-hydrate analysis. Two ions, m/z 369 (C27H45+) and m/z 385 (C27H45O+), are the characteristic peaks of cholesterol, known as [M+H–H2O]+ and [M−H]+, respectively. In ToF–SIMS imaging, the rat brain was cut in the sagittal direction. Negative ion images obtained at the cerebellum region prepared by using f thaw-mounted rat brain on a stainless steel plate and room temperature-drying, g tape-supported rat brain and freeze-drying, and h tape-supported rat brain and frozen-hydrated analysis. Scale bar is 0.5 mm. MC stands for maximal counts

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