Fig. 5
(Reproduced from ref. [91] with permission, © American Chemical Society)
Schematic of the workflow for lipid imaging with SIMS following exposure to TFA vapor. Tissue slices on microscope slides with an electrically conductive coating (indium tin oxide, ITO) were placed in a desiccator containing 300 μL of TFA for different lengths of time. The samples were transferred to the mass spectrometer for SIMS analysis where the tissue was bombarded with a focused beam of Ar4000+ ions. Several hundred peaks with distinct mass signals were detected of which selected lipid species were imaged