Fig. 5From: Probing the subcutaneous absorption of a PEGylated FUD peptide nanomedicine via in vivo fluorescence imagingIsothermal titration calorimetry (ITC) was used to determine the binding affinity (Kd) and other binding parameters of the interaction between the sulfo-Cy5 labeled peptide and human plasma fibronectin. Shown here are isotherms and thermographs of experiments involving a FUD-Cy5 or b 20 kDa PEG-FUD-Cy5 interaction with FN. All experiments were performed in duplicates using pH 7.4 PBS at 25 °CBack to article page