Fig. 1From: Multi-modal liquid biopsy platform for cancer screening: screening both cancer-associated rare cells and cancer cell-derived vesicles on the fabric filters for a reliable liquid biopsy analysisThe effect of CB on cell viability and EV secretion: a precisely controlling the CB concentration and treatment time enabled multi-modal screening of CTCs. CB treatment promoted cancer cells captured on the fabric filters to secrete large number of EVs with minimal cytotoxic effect. b Viability of CB-treated cancer cells were measured using two-colored live/dead assay. c Viability of cancer cells were further quantitatively measured in depth, using Alamar blue viability assay after 3 h-CB treatment. No significant cytotoxic effects were found at the CB concentration of ≤ 10 µg/mL and treatment time of ≤ 3 h. d SEM was utilized to image the EVs secreted from MCF-7 cancer cells. e, f CB treatment significantly enhanced the EV secretion. CB increased EV secretion by 3.5-fold at the concentration of 5 μg/mL and there was no further significant increment in EV concentration until it reached 20 μg/mLBack to article page