Table 1 Comparison of recent PfLDH detection studies
Diagnosis | Target | Bio-recognition | Output | LOD | Volume | References |
|---|---|---|---|---|---|---|
Microfluidic microplate | PfLDH in buffer | Antibody | Fluorescence intensity | 0.025 pg/μL | 5 μL | This study |
Microfluidic microplate | PfLDH in human serum | Antibody | Fluorescence intensity | 1 pg/μL | 5 μL | This study |
ELISA | PfLDH in buffer | Antibody | Absorbance | 0.1 pg/μL | 100 μL | This study |
Lateral flow immunoassay | PfLDH in buffer | Antibody | Colorimetric assay | 10 pg/μL | 30 μL | [31] |
AuNPs based aptasensor | PfLDH in lysed RBCsa solution (Mimic real sample) | Aptamer | Colorimetric assay | 38 pg/μL | 10 μL | [32] |
MNP-Qdot aptasensor | PfLDH in buffer | Aptamer | Fluorescence intensity | 0.0066 pg/μL | 100 μL | [33] |
Aptamer-tethered enzyme capture (APTEC) assay | PfLDH in buffer | Aptamer | Colorimetric assay | 4.9 pg/μL | 10 μL | [34] |
Aptamer-tethered enzyme capture (APTEC) assay | PfLDH in serum | Aptamer | Colorimetric assay | 50 pg/μL | 40 μL | [35] |