Fig. 4From: Regulation of intracellular transition metal ion level with a pH-sensitive inorganic nanocluster to improve therapeutic angiogenesis by enriching conditioned medium retrieved from human adipose derived stem cellsThe effect of endocellularly released iron through endocytosis. TEM image of hADSCs (a) with and (b) without ps-TNC treatment (3 µg/mL, 12 h, N: nucleus, red arrows: ps-TNCs in endosomes, Scale bars = 2 µm). Dash lined box shows the high magnification image in the left side. c Comparison of HIF-1a, hFGF and VEGF expression in untreated hADSCs, with gold nanoparticles treatment (3 µg/mL, 12 h), and ps-TNC treatment (3 µg/mL 12 h, n = 3, #p < 0.01, *p < 0.05 compared to the untreated group). d Relative amount of iron ion detected in hADSCs after ps-TNC treatment as quantified by ICP-OES (n = 5, *p < 0.01 compared to the untreated group). e The amount of iron ion detected in various media as quantified by ICP-OES (n = 4). f, g The migration of hADSCs treated with CM or ps-TNC-CM for 24 h (n = 4, #p < 0.01, ##p < 0.001 compared to 0 h of each groups)Back to article page