Fig. 5
From: Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system
a The live/dead assay confocal microscopic images of liver MPS and liver fibrosis-on-chip model with different ECM. The green color is representative of live cells, while the red color is showing the dead cells. These confocal microscopy images of the live/dead assay were further analyzed with ImageJ (image processing software), and the results were displayed as a percentage (scale bar is 200 µm). b The live/dead assay graph shows the cell viability of control and liver fibrosis-on-chip model with different ECM. Fibronectin based liver MPS showed 90% cell viability, while 88% cell viability was observed for collagen and poly-l-lysine based liver MPS. In the liver fibrosis-on-chip model, the cell viability reduced to 77%, 76%, and 73% for fibronectin, collagen, and poly-l-lysine (data presented as mean ± SD, *represent statistical significance from the control, while the p < 0.05). c CYP3A4 expression in control MPS and liver fibrosis-on-chip model with different ECM (data presented as mean ± SD)