Fig. 6
From: Ultrasmall iron oxide nanoparticles induced ferroptosis via Beclin1/ATG5-dependent autophagy pathway
Reversal effect on the USIONPs-induced ferroptosis by the interference of Beclin 1/ATG5 for 48 h. A-a Inhibitory rates of U251 cells after silencing of Beclin1 gene; A-b Concentrations of iron in the U251 cells after silencing Beclin1 gene; A-c Relative levels of lipid ROS in the U251 cells after silencing Beclin1 gene; A-d Relative levels of ROS in the U251 cells after silencing Beclin1 gene; A-e Representative images of expression of autophagy and ferroptosis related proteins in the U251 cells after silencing Beclin1 gene; A-f Relative expression of autophagy and ferroptosis related proteins in the U251 cells after silencing Beclin1 gene; B-a Inhibitory rates of U251 cells after silencing ATG5 gene; B-b Concentrations of iron in the U251 cells after silencing ATG5 gene; B-c Relative levels of lipid ROS in the U251 cells after silencing ATG5 gene; B-d Relative levels of ROS in the U251 cells after silencing ATG5 gene; B-e Representative images of expression of autophagy and ferroptosis related proteins in the U251 cells after silencing ATG5 gene; B-f Relative expression of autophagy and ferroptosis related proteins in the U251 cells after silencing ATG5 gene. *P < 0.05, statistically significant compared with control group. #P < 0.05, statistically significant compared with 200 μg/mL USIONPs