Fig.7
From: Ultrasmall iron oxide nanoparticles induced ferroptosis via Beclin1/ATG5-dependent autophagy pathway
Stimulation of USIONPs-induced ferroptosis by the overexpression of Beclin 1/ATG5. A-a Inhibitory rates of U251 cells after overexpression of Beclin1 gene for 48 h; A-b Concentrations of iron in the U251 cells after overexpression of Beclin1 gene for 48 h; A-c Relative levels of lipid ROS in the U251 cells after overexpression of Beclin1 gene for 48 h; A-d Relative levels of ROS in the U251 cells after overexpression of Beclin1 gene for 48 h; A-e Representative images of expression of autophagy and ferroptosis related proteins in the U251 cells after overexpression of Beclin1 gene for 48 h; A-f Relative expression of autophagy and ferroptosis proteins in the U251 cells after overexpression of Beclin1 gene for 48 h; B-a Inhibitory rates of U251 cells after overexpression of ATG5 gene for 48 h; B-b Concentrations of iron in the U251 cells after overexpression of ATG5 gene for 48 h; B-c Relative levels of lipid ROS in the U251 cells after overexpression of ATG5 gene for 48 h; B-d Relative levels of ROS in the U251 cells after overexpression of ATG5 gene for 48 h; B-e Representative images of expression of autophagy and ferroptosis proteins in the U251 cells after overexpression of ATG5 gene for 48 h; B-f Relative expression of autophagy and ferroptosis related proteins in the U251 cells after overexpression of ATG5 gene for 48 h. *P < 0.05, statistically significant compared with control group. #P < 0.05, statistically significant compared with 200 μg/mL USIONPs