Fig. 8
From: Ultrasmall iron oxide nanoparticles induced ferroptosis via Beclin1/ATG5-dependent autophagy pathway
USIONPs-induced ferroptosis influenced by lysosome inhibitors for 48 h. a Inhibitory rates of U251 cells in exposure to USIONPs with or without lysosome inhibitors (ConA, BAFA1, and CQ); b Concentrations of iron in the U251 cells in exposure to USIONPs with or without lysosome inhibitors; c Relative levels of lipid ROS in the U251 cells in exposure to USIONPs with or without lysosome inhibitors; d Relative levels of ROS in the U251 cells in exposure to USIONPs with or without lysosome inhibitors; e Representative images of expression of autophagy and ferroptosis related proteins in the U251 cells in exposure to USIONPs with or without lysosome inhibitors; f Relative expression of autophagy and ferroptosis related proteins in the U251 cells in exposure to USIONPs with or without lysosome inhibitors.*P < 0.05, statistically significant compared with control group. #P < 0.05, statistically significant compared with 200 μg/mL USIONPs