Skip to main content
Fig. 2 | Nano Convergence

Fig. 2

From: Probing mechanobiological role of filamin A in migration and invasion of human U87 glioblastoma cells using submicron soft pillars

Fig. 2

Cell spreading and rigidity sensing ability of NC and FLNa-KD cells on fibronectin-coated pillars (diameter, 900 nm; height, 2 μm; and pillar center-to-center distance, 1.8 μm; bending stiffness k = 24.2 nN/µm). A Western blot of FLNa. Control: GAPDH. B Fluorescence images of F-actin (RP: red) and the nuclei (DAPI: blue) of cells on fibronectin-coated pillars at 1 and 6 h. C Aspect ratio of cells. (n = 30 cells). D Deflection of pillars in NC and FLNa-KD cells near the edge (approximately 34.5 µm2) of cells at < 30, 60, and 360 min incubations. Local contractions in the NC cells are marked with cyan circles. The red arrows indicate pillar deflection. E Directionality parameters (γ) of the NC and FLNa-KD cells at < 30, 60, and 360 min incubations. γ is calculated as the sum of the force vectors of pillars divided by the sum of their magnitudes. γ is 1 when the force vectors of two neighboring pillars are parallel. γ is 0 when the force vectors of the pillars are locally contracted toward each other. Cell number n = 6. Error bar represents the mean ± standard deviation (SD). *** P < 0.001. Student’s t-test. NS Not significant

Back to article page