Fig. 5

Invasiveness and filopodia formation of NC and FLNa-KD cells. A Number of invasive cells obtained from 2 × 10⁴ cells initially seeded on the BioCoat™ Matrigel® Invasion Chamber for 24 h. Each experiment was repeated three times. Error bars represent the mean ± SD of experiments. ** P < 0.01; Student’s t-test. B Images of filopodia extension in cells with fluorescent staining of F-actin (RP: red), nuclei (DAPI: blue), and immunofluorescent staining of the actin bundling protein fascin (green)