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Nano Convergence
Scheme 1 | Nano Convergence

Scheme 1

From: Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities

Scheme 1

Schematic of the synthetic route of the antibody-conjugated NPs. (Upper) Synthesis of the full-CD8a NPs. The EDC activates the COOH group of the PLGA NPs, and the NHS subsequently forms a stabilized amine-reactive sulfo-NHS ester. This allows the NH2 group of the antibody to bind with the amine-reactive sulfo-NHS ester on the NP surface. (lower) Synthesis of the f(ab′)2-NPs. An antibody was first cleaved by using IdeS. It was then reduced by DTT to obtain a free thiol group in its hinge region. The free thiol group binds with the Mal located on the NP surface

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