Table 1 The amounts of anti-CD8a Ab conjugated to the PLGA-PEG-COOH NPs. The amounts (µg) of antibodies conjugated to 1 mg of the PLGA-PEG-COOH NPs when antibodies were initially added at 25 µg per 1 mg of NPs was measured by BCA assay (n = 3, mean ± SD)
Coupling time (h) | Activation time (h) | |||
|---|---|---|---|---|
3 | 6 | 10 | ||
3 | 1.74 ± 0.39 | 3.48 ± 0.44 | 2.90 ± 0.59 | |
6 | 2.42 ± 0.72 | 3.61 ± 0.50 | 3.41 ± 1.00 | |
10 | 3.36 ± 0.40 | 3.86 ± 0.32 | 5.25 ± 0.30 | |