Table 2 The amounts of anti-CD8a Ab conjugated to the PLGA-PEG-COOH NPs. The amounts (µg) of antibodies conjugated to 1 mg of the PLGA-PEG-COOH NPs when antibodies were initially added at 50 µg per 1 mg of NPs was measured by BCA assay (n = 3, mean ± SD)
Coupling time (h) | Activation time (h) | |||
|---|---|---|---|---|
3 | 6 | 10 | ||
3 | 3.56 ± 0.58 | 4.82 ± 0.29 | 4.34 ± 0.66 | |
6 | 5.28 ± 0.49 | 4.82 ± 0.50 | 6.31 ± 0.35 | |
10 | 5.28 ± 0.75 | 5.55 ± 0.27 | 7.32 ± 0.95 | |