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Nano Convergence
Fig. 2 | Nano Convergence

Fig. 2

From: Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation

Fig. 2

Confirmation of the effect of nano- and micropatterns for cell behaviors. a FE-SEM images of PR nanohole patterns and PDMS nanopillar patterns, and b size distribution of PDMS patterns. c 3D SEM image of PDMS nanopillar patterns. d Confocal microscope images of the cells on bare PDMS, and 400, 600, and 800 nm PDMS patterns (left to right) immunostained with actin (Red) and Hoechst (Blue). e−g Cell spreading area (e), circularity (f), and cell aspect ratio (g) of the cells on bare PDMS, and 400, 600, and 800 nm PDMS pattern. h FE-SEM images of micropatterned silicon wafer, PDMS and side view image of micropatterned PDMS. i Confocal microscope images of the cells on bare PDMS and micropatterned PDMS immunostained with actin (Red) and Hoechst (Blue). j Cell orientation analysis of the cells on the bare PDMS and micropatterned PDMS. (*** p ≤ 0.001)

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