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Fig. 4 | Nano Convergence

Fig. 4

From: Nano-sized graphene oxide coated nanopillars on microgroove polymer arrays that enhance skeletal muscle cell differentiation

Fig. 4

Investigation of the cell behaviors on the GO-coated NMPA. a FE-SEM images of micro-nano pattern on silicon wafer and NMPA. b Raman intensity map of the LGO-, 10-sGO-, and 5-sGO-coated micropattern, nanopattern, and NMPA. c Confocal microscope images of the cells on bare PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA immunostained with actin (Red) and Hoechst (Blue). d The cell orientation graph of the cells on the bare PDMS, micropatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. e Cell alignment index of the cells on bare PDMS, micropatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. fh Cell spreading area (f), circularity (g), and cell aspect ratio (h) of the cells on the bare PDMS, micropatterned PDMS, nanopatterned PDMS, NMPA, LG-NMPA, 10-sG-NMPA, and 5-sGO-NMPA. (* p ≤ 0.5, *** p ≤ 0.001)

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