Fig. 4
From: Bioenzyme-based nanomedicines for enhanced cancer therapy
Reproduced with permission from [59]. Copyright 2018, Wiley–VCH
a Chemical structures of PCB1 and PCB2 and schematic for the synthesis of PCB-Bro. b Immunofluorescence collagen I staining images of 4T1 tumors after intratumoral injection of saline, PCB1, or PCB1-Bro with or without laser irradiation. Cell nucleus were stained by 4′,6-diamidino-2-phenylindole (DAPI) and collagen I was stained by Alexa Fluor 488 conjugated anti-collagen I antibody. c Illustration of photothermally triggered enzyme activation of PCB1-Bro towards collagen digestion for enhanced accumulation of nanoparticles in tumors. d Tumor growth curves of 4T1 tumor-bearing mice in different groups.