Fig. 1

The human gut-on-a-chip with microelectrode arrays. A Schematic of the gut-on-a-chip. The left channel was used to establish an intestinal lumen using human epithelial Caco-2 cells. The right channel was employed to make a vascular lumen using HUVECs. The continuous flow of the culture medium was introduced by an osmotic pump. B Representative immunofluorescence results showing that ZO-1-positive Caco-2 cells and PECAM-1-positive HUVECs were located in the left and right channel of a gut-on-a-chip separated by collagen type I gels. Scale bar is 100 μm