Fig. 1

Overview of chip design and hydrogel/media patterning. A A photograph of the MV-IMPACT chip body with top lid. B Rendering of a single unit well with cross section view of the entire well and patterned gel. C Illustration of edge guided sequential patterning within a cross section view of a unit well. D Different methods of inducing flow conditions. From left to right, levelling flow utilizes differences in media volumes within the two reservoirs to induce flow through the patterned gels via equilibration. Tilt induced flow elevates one reservoir over another to facilitate flow. Static flow conditions can be induced by adding the same volume of media in both reservoirs