Fig. 4

Angiogenic assays conducted testing for stromal component mediated and DAPT induced notch inhibition mediated morphological differences. A Confocal images of angiogenic sprouting induced by stromal cell component groups comprising of normal human lung fibroblasts at 6 × 10⁶ cells mL⁻¹ and 3 × 10⁶ cells mL⁻¹ seeding concentrations, 6 × 10⁶ cells mL⁻¹ SW620, 3 × 10⁶ cells mL⁻¹ SW620 + 3 × 10⁶ cells mL⁻¹ LF, 6 × 10⁶ cells mL⁻¹ HEPG2, and 3 × 10⁶ cells mL⁻¹ HEPG2 + 3 × 10⁶ cells mL⁻¹ LF. B Charts the top down vessel areas relative to the 3 × 10⁶ cells mL⁻¹ LF control. C Confocal images of angiogenic sprouting under untreated control (EGM-2 media only), DMSO control (EGM-2 media with 0.25% DMSO by volume), and DAPT treatment (EGM-2 media with 25 µM DAPT and 0.25% DMSO by volume). D, E Chart visualizing mean vessel width, branching, and mean vessel length between treatment groups. The quantification of angiogenic sprouting was performed after 7 days of cultivation within the chip (*p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.00005 and n.s. not significant)