Fig. 3
From: Nanotechnology-enabled immunoengineering approaches to advance therapeutic applications
Reproduced with permission from ref [47]
Lipid calcium phosphate nanoparticle (LCP NP)-mediated co-delivery of Trp2 peptide and CpG in B16F10 subcutaneous tumor. a Synthesis LCP NPs that encapsulate phosphorylated Trp2 (p-Trp2) and CpG for the delivery into DCs via the mannose receptor. TEM image of b hydrophobic LCP cores and c mannose-functionalized, aqueous LCP NPs. In vivo CTL response assay examining mice immunized with control peptide/CpG or mannose-LCP NPs containing both Trp2/CpG against splenocytes pulsed with d Trp2 or e p-Trp2. f, g Reduction of B16F10 tumor sizes in mice immunized with LCP NPs containing both Trp2 and CpG.