Fig. 5
From: Nanotechnology-enabled immunoengineering approaches to advance therapeutic applications
Reproduced with permission from ref [99]
Polymeric nanocarrier for the delivery of CAR IVT mRNA into T cells. a Schematic illustration of the PBAE nanocarrier encapsulating the IRF5 and IKK IVT mRNA. The polymeric nanoparticles created from self-assembly could be readily lyophilized and redispersed, increasing flexibility for storage and transport. c Transfection efficiencies of the nanocarriers into T cells. d The nanoparticle-reprogrammed T cells showed comparable lysis activity to lentivirus-transduced T cells against Raji lymphoma cells. e Only the transfected T cells were able to alleviate tumor burden and f extend average survival time in C57BL/6 mice.