Fig. 12
(Reprinted/adapted by permission from Springer Nature: Sun et al. [66], copyright 2021)
Click-ExM imaging of proteins, nucleic acids and small molecules. a Chemical structure of EdU, and pre- and post-click-ExM images of nascent DNA (AF488) in EdU-treated HeLa cells. Scale bar, 5 μm. b Chemical structure of EU, and pre- and post-click-ExM images of nascent RNA (AF555) in EU-treated HeLa cells. Scale bar, 5 μm. c Chemical structure of Az-afatinib, and pre- and post-click-ExM images of HeLa cells treated with Az-afatinib (AF555). Scale bars, 10 μm. d Chemical structure of OP-Puro, and pre- and postc-lick-ExM images of nascent peptides (AF488) in OP-Puro-treated HeLa cells. Scale bar, 10 μm. e Chemical structure of Alk-Hoechst, and pre- and post-click-ExM images of DNA (AF488) in Alk-Hoechst-labeled HeLa cells. AcX was used for anchoring. Scale bar, 5 μm. All the scale bars are in the pre-expansion dimension.