Fig. 14

ExM variants with higher expansion factors. a Single-round expansion with high expansion factor (~ 10×). The chemical composition of the hydrogel is schematically illustrated for Ten-fold Robust Expansion Microscopy (TREx, left) and X10 Expansion Microscopy (right). b Iterative expansion. First, the sample is embedded in an expanding hydrogel (blue). Next, the expanded sample-hydrogel composite is re-embedded in a second expanding hydrogel (orange) and expanded once again