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Nano Convergence
Fig. 2 | Nano Convergence

Fig. 2

From: Bolstering the secretion and bioactivities of umbilical cord MSC-derived extracellular vesicles with 3D culture and priming in chemically defined media

Fig. 2

Characterizations of EV from three different culture conditions. a ZetaView® analysis for the size and number of total particles of 2D CDM EV and 3D CDM EV. b The number of particles per cell for 2D CDM EV and 3D CDM EV. c The Western blot analysis of 2D CDM EV and 3D CDM EV for representative surface markers of EVs. d The benefits of MSC activation using TNF-α and IFN-γ (TI) priming. e Time-dependent cell viability with TI activation in NM and CDM (NM; Normal media, 10% fetal bovine serum supplemented ɑ-MEM, CDM; CellCor™ CD MSC media). f Comparison of cell viabilities in TI-activated hUCMSC in NM and CDM. g The expression level of HGF in culture media of hUCMSC. h The total number of particles derived from different concentrations of TI treatments for hUCMSC spheroids in CDM. i The number of particles per cell with and without TI priming in 3D culture. j The size and total number of particles for TI3D CDM EV were analyzed using ZetaView®. k The Western blot analysis for representative surface markers of TI3D CDM EV. l The morphologies of EVs are characterized by TEM. Scale bars equal to 100 nm. (Values are presented as mean ± SD (n = 3) and statistical significance was obtained with one-way analysis of ANOVA with Tukey’s multiple comparison post-test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001))

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