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Nano Convergence
Fig. 3 | Nano Convergence

Fig. 3

From: Self-assembled, hemin-functionalized peptide nanotubes: an innovative strategy for detecting glutathione and glucose molecules with peroxidase-like activity

Fig. 3

a Schematic depiction of hemin-PNTs as a nanoenzyme. b The UV‒vis absorption spectra of hemin-PNTs + TMB + H2O2 change as the reaction time increases. The concentrations of hemin-PNTs, TMB, and H2O2 were 3.3 μg/mL, 3 mM, and 10 mM, respectively. c Changes in the absorbance of TMB solution over time at 652 nm in the following reaction systems: a. TMB + H2O2; b. hemin-PNTs + TMB; c. PNTs + TMB + H2O2; d. hemin + TMB + H2O2; e. hemin@PNTs + TMB + H2O2; and f. hemin-PNTs + TMB + H2O2. d Absorption spectra of TMB–H2O2 mixed solution in the absence of a catalyst (black line) and in the presence of 3.3 μg/mL PNTs (green line), 3.3 μg/mL hemin (blue line), 3.3 μg/mL hemin@PNTs (green line) and 3.3 μg/mL hemin-PNTs (red line). e Changes in the absorbance of a 10 mM H2O2 and 3 mM TMB solution are shown at different hemin-PNT concentrations. f The catalytic activity of hemin, PNTs, hemin@PNTs, and hemin-PNTs at various concentrations of H2O2. g TMB, OPD, and ABTS absorption spectra changed as a result of hemin-PNT-catalyzed oxidation. Data represent means ± SDs (n = 3)

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