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Nano Convergence
Fig. 5 | Nano Convergence

Fig. 5

From: Intracellular lipophilic network transformation induced by protease-specific endocytosis of fluorescent Au nanoclusters

Fig. 5

Competitive binding inhibition of bPAuNC and pPAuNC by increasing extracellular concentration of albumin in MDA-MB-231 cells. For binding inhibition, MDA-MB-231 cells were incubated with 2% albumin for 4 h; non-treated (w/o) and treated (w/). After albumin blocking, a bPAuNC and b pPAuNC were treated to MDA-MB-231 cells for 10 h; nucleus (blue), lipophilic membrane dye (CLLD, pseudocolor: green), bPAuNC or pPAuNC (red) and co-localization of CLLD and bPAuNC or pPAuNC (yellow). Scale bars are 50 μm. c, e Average fluorescence intensity of bPAuNC or pPAuNC in the cell cytoplasm. d, f Scatterplots and bar graphs through co-localization analysis of CLLD against bPAuNC or pPAuNC, respectively. Pearson’s coefficient and correlation values were generated using the ImageJ program; Slope, Rcoloc, %Volume, and Ncoloc

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