Fig. 5
From: 3D printed fluidic swab for COVID-19 testing with improved diagnostic yield and user comfort
LFA and RT-qPCR detection for SARS-CoV-2 in NF and PNS collected from the 3D printed human nose by the 3DPFS and CS. A The 3D printed human nose and collection of NF or PNS from the nose by NF and PNS. B Schematic describing sample loading and collection of the sample in the nose by a pipette and either CS or 3DPFS, respectively. 100 µL of SARS-CoV-2 lysates-spiked NF at different concentrations (101–104 pfu/mL) or COVID-19 PNS was loaded into the nose by a pipette. C The results of LFA for the collected SARS-CoV-2-spiked NF from the nose 3DPFS and CS. Quantitative analysis of the test l’ne’s color intensity relative to that of the control line, using ImageJ. Student’s t-test was used to compare the data obtained under different conditions. D The results of RT-qPCR analysis for the collected PNS samples from the nose by 3DPFS and CS. Data with a p-value less than 0.05 were considered significant (*p < 0.05, **p < 0.01, ***p < 0.001). n = 3