Nano-biomaterials and advanced fabrication techniques for engineering skeletal muscle tissue constructs in regenerative medicine

Han, Seokgyu; Cruz, Sebastián Herrera; Park, Sungsu; Shin, Su Ryon

doi:10.1186/s40580-023-00398-y

Nano Convergence

Table 5 Bioprinting for generation of functional skeletal muscle construct

From: Nano-biomaterials and advanced fabrication techniques for engineering skeletal muscle tissue constructs in regenerative medicine

Printing methods	Materials	Cells	Animal model	Features	Refs.
Inkjet printing	Fib/BMP-2	hMPCs	–	Controlling cells fate of primary muscle derived stem cells toward osteogenic or myogenic cells by BMP-2 patterning	[118]
3D ITOP	Fib/gelatin/ HA/glycerol	hMPCs	Rat VML TA	Fabricating biomimetic implantable human skeletal muscle construct (mm³-cm³ size) made up of hundreds of long parallel myofiber bundles	[120]
	Fib/gelatin/ HA/glycerol	hMPCs/hNSCs	Rat VML TA	Forming neuro-muscular junction and facilitating rapid innervations in rodent model	[121]
	Fib/NF loaded microsphere	hMPCs	–	Accelerating peripheral nerve regeneration and innervation by release NF delivery	[122]
Extrusion	Au nanowires/collagen	C2C12	Rat VML temporalis muscle	Increasing C2C12 differentiation by aligned in Au nanowires	[130]
	Collagen	hADSC/HUVEC	Rat VML temporalis muscle	Achieving in-situ direct bioprinting by combining bioprinting with bioreactor enabling	[145]
	dECM	–	Canine VML biceps femoris	Acellular 3D bioprinted dECM patches for implantation of bulk patient-specific scaffold (12 × 8 × 2 cm) based on computed tomography imaging	[127]
	Collagen	hMSCs	Rat VML TA	Fabricating collagen microfiber with 1,000 times higher tensile strengths than a normal collagen gel	[124]
SLA	Oxidized methacrylic alginate/PEGMA	Primary neurons/C2C12	–	Demonstrating spatial control of neurons and myoblasts for enhancing functionality of neurons	[125]
DLP	Poly (glycerol sebacate) acrylate	C2C12	Rat VML TA	Tuning of mechanical and geometrical cues through 3D printing process	[126]
Embedded printing	GelMA	hiPSC-MPCs/HUVEC	Mouse VML quadriceps	Forming endothelialized perfusable channels with increasing iPSC-MPC’s viability and functionality	[123]

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