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Fig. 2 | Nano Convergence

Fig. 2

From: Recombinant protein embedded liposome on gold nanoparticle based on LSPR method to detect Corona virus

Fig. 2

Preparation and Characterization of ACE2 and R/Li. A Confirmation of vector and insert by ACE with YFP. B Western blot for recombinant protein expression image of ACE2. (Before/After purification) (C) Size distribution of DLS depending on sonication time and extrusion of liposomes and R/Li. D Result of representation ACE2 in membrane by ChARMM-GUI. (Inlet = Actual representation of ACE2 in liposome) The binding domain with SARS-CoV-2 S1 protein was shown as red. E Simulation of ACE2 with POPC and POPG by AutoDock. F Cryo-EM images of liposomes (left) and R/Li (right). (Scale bars = 20 nm) G Confocal microscope image of R/Li with YFP (yellow fluorescence protein). Scale bars = 200 nm (Arrow = receptor)

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