Fig. 2

Characterization of DNase-NZ. a–d Size distribution, average diameter, and polydispersity index of a PLGA NPs, b Dopa@PLGA NPs, c PEG@D-PLGA NPs, and d DNase-NZ. a–d Insets represent a scanning electron micrograph of each particle imaged at 10,000× magnification. Scale bar: 200 nm. e Surface charges of PLGA NPs, Dopa@PLGA NPs, PEG@D-PLGA NPs, and DNase-NZ. f The enzymatic activity of DNase-NZ was confirmed using gel electrophoresis. g The enzymatic activities of DNase-NZ were successfully preserved after prolonged incubations (1, 3, 6, 12, 24, and 48 h) at 37 ℃. h In vitro cytotoxicity of DNase-NZ on L929 cells at various concentrations (0.5, 1, 2, 5, 10, 100, 500, and 1000 μg/mL) after 24 h incubation. i NET-degrading ability of DNase-NZ was confirmed after incubation with NETosis-induced bone marrow derived neutrophils (BMDNs). Data represented as mean ± SD. e, h, i Statistical significance was assessed using a two-tailed Student’s t-test. ***p < 0.0002, ****p < 0.0001