Fig. 4

Changes in immune cell population and NETosis-related genes after treatment with DNase-NZ. a Representative flow cytometry plots (left) of leukocytes as identified by CD45⁺ cells in the lamina propria of mice (gated on live cells). Numbers within the plot indicate the average percentage of cells in the corresponding subset. The bar graph (right) represents the percentage of cells positive for CD45. (n = 5) b Representative FACS plots (left) of the neutrophils as identified by Ly6G and CD11c in the lamina propria of mice (gated on CD45 + leukocytes). Numbers within the plot indicate the average percentage of cells in the corresponding subset. The bar graph (right) represents the percentage of cells positive for Ly6G and CD11c. c–e Relative mRNA expression levels of genes, including c Cxcl5, d Elane, and e Padi4, were determined using quantitative PCR assay (n = 5). Gapdh was used as an internal reference and relative expression levels were calculated against control mice. All assays were performed in at least duplicates. Data are presented as mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-test. *p < 0.0332, **p < 0.0021, ***p < 0.0002, ****p < 0.0001