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Optimization and stabilization of gold nanoparticles by using herbal plant extract with microwave heating

Nano Convergence volume 1, Article number: 12 (2014) Cite this article

Abstract

In this study, we have synthesized the gold nanoparticles by using Hibiscus rosa-sinensis, a medicinal plant. The gold nanoparticles were synthesized rapidly by the involvement of microwave heating. By changing of plant extract concentration, gold solution concentration, microwave heating time and power of microwave heating the optimized condition was identified. The surface Plasmon resonance found at 520 nm confirmed the gold nanoparticles synthesis. The spherical sized nanoparticles in the size range of 16–30 nm were confirmed by Transmission Electron Microscope (TEM). The stability of the nanoparticles is very well proved in the invitro stability tests. The biochemical like alkaloids and flavonoids play a vital role in the nanoparticles synthesis was identified using the Fourier Transform Infrared Spectroscopy (FTIR). Combining the phytochemical and microwave heating, the rapid synthesis of gold nanoparticles is the novel process for the medically applicable gold nanoparticles production.

Background

Nanotechnology is mainly concerned with the synthesis of nanoparticles of variable sizes, shapes, chemical compositions and controlled dispersity and their potential use for biomedical applications [1]. Although chemical and physical methods may successfully produce pure, well defined nanoparticles, these are quite expensive and potentially dangerous to the environment.

As an alternative to toxic and expensive physical methods for nanoparticles fabrication, using microorganisms, plants and algae will help a lot to synthesize the materials in the nano range and in addition, the toxicity of the by-product would be lesser than the other synthetic methods [2–4]. Many of the scientists are strongly supporting that there will not be any release of toxic substances during the nanoparticles synthesis with the help of green materials. The cause is chemicals which will be used in nanoparticles synthesis will get degraded by the enzymatic substances which are produced by the microbes during the time of growth. Plants also by trapping the bio-chemical materials with in their parts use the same as nutritive materials for metabolic processes of their own [5]. Using the biological organisms such as micro organisms [6], plant extract or plant biomass could be an alternative to chemical and physical methods for the production of nanoparticles [7, 8].

Stabilization of gold nanoparticles using phyto-synthesis and microwave heating techniques is an emerging area in the field of advanced nanoparticles synthesis. Several plants and plant products have been successfully used for efficient and rapid extracellular synthesis of silver and gold nanoparticles. Leaf extracts of Coleus aromaticus[9], Garcinia mangostana[10], Barbated skullcup herb extract [11], Magnolia kobus and Diopyros kaki leaf extracts [12], Rosa hybrid petal extract [13], Nyctanthes arbortristis ethanolic flower extract [14], Cassia fistula leaf [15], Lippia citriodora (Lemon verbena) leaves extract [16] have also been used for gold nanoparticles synthesis. Using plants and plant materials for synthesis of metal nanoparticles could be advantageous over other environmentally benevolent biological processes by eliminating the elaborate process of maintaining bacterial and fungal cultures. It can also be easily scaled up for large scale production of beneficial nanoparticles.

A well known member of the family Malvaceae, Hibiscus rosa-sinensis grows as an important herbaceous plant. The bio-chemical constituents of the plants are Taraxery acetate, β-sitosterol, campesterol, stigmasterol, cholesterol, erogosterol, lipids, critic, tartaric and oxalic acids, fructose, glucose, sucrose, falvonoids and flavonoid glycosides [17], Hibiscentin, cyaniding and cyanin glucosides are alkanes. This plant has lot of applications like anti-infectious, anthelmintic, anti-inflammatory, diuretic, antipyretic [18]. The young leaves and flowers are used in inducing abortion and as a cure for headache. The plant has many hidden medical benefits [19].

In this study we synthesized the optimized gold nanoparticles by using medicinal plant extract Hibiscus rosa-sinensis. For the optimized production of gold nanoparticles, five different concentrations of Hibiscus rosa-sinensis leaf extract was added with gold chloride solution in different microwave heating voltage and time intervals. After the synthesis, the nanoparticles were confirmed by UV–vis spectrophotometry, Transmission Electron Microscopy and the stability of nanoparticles also measured by UV–vis spectroscopy.

Methods

Materials

The Hibiscus leaves for analysis were collected from Vellore District, South India. The chlororauric acid (HAuCl4), cysteine, Sodium chloride and sodium phosphate were purchased from Hi Media, Mumbai.

Green synthesis of optimized gold nanoparticles

2, 4, 6, 8 and 10 g of Hibiscus rosa-sinensis leaves cut into appropriate size was taken to in 100 ml wide neck borosil conical flask and washed several times with deionised water. 100 ml deionised water was added to the flask containing freshly cut Hibiscus rosa-sinensis leaves exposed to microwave heating for 3 min. The resultant crude extract was filtered with Whatman filter paper No. 40 and used for synthesis process. 1 mM of gold chloride solution was taken for the gold nanoparticles synthesis.

Optimization of gold nanoparticles synthesis was done by changing the parameters like Plants extracts concentration, temperature (Power of microwave heating) and Microwave heating time. The details of the optimization parameters are given in Tables 1, 2, 3, 4 and 5.

Table 1 Preparation of gold nanoparticles by 2% (2 ml) leaf extracts concentration
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Table 2 Preparation of gold nanoparticles by 4% (4 ml) leaf extracts concentration
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Table 3 Preparation of gold nanoparticles by 6% (6 ml) leaf extracts concentration
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Table 4 Preparation of gold nanoparticles by 8% (8 ml) leaf extracts concentration
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Table 5 Preparation of gold nanoparticles by 10% (10 ml) leaf extracts concentration
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Characterization of gold nanoparticles

Preliminary characterization of gold nanoparticles was carried out using UV–vis spectroscopy (Tecan plate reader-infinite m200 model). Gold nanoparticle powder sample was prepared by centrifuging the synthesized gold nanoparticle solution at 10,000 rpm for 15 min for Fourier transformed infrared radiation spectroscopy measurement. The solid residue layer which contains gold nanoparticles was dispersed in sterile deionised water three times to remove the attached biological impurities. The pure residue was then dried in an oven overnight at 65°C. The obtained powder was subjected to FT-IR measurement carried out on a Perkin Elmer spectrum-one instrument at a resolution of 4 cm−1 in KBr pellets. Transmission electron microscopy measurement of synthesized gold nanoparticles using Hibiscus rosa-sinensis leaf extract were carried out on Techni-20 Philips transmission electron microscope operated at 80 kev. TEM sample were prepared by the dispersion of 2–3 drops of Hibiscus rosa-sinensis stabilized gold nanoparticle solution on a copper grid and dried at room temperature after the removal of excess solution with filter paper.

In-vitro stability studies of synthesized gold nanoparticle using Hibiscus rosa-sinensis leaves

In-vitro stability study of optimized Hibiscus rosa-sinensis stabilized gold nanoparticle was tested in the presence of 10% NaCl, 0.2 M Cysteine, PBS 6, PBS 7.4 and PBS 8. Typically, 1 ml of gold nanoparticle solution was added to 24 well plates containing 0.5 ml of 10% NaCl, 0.2 M Cysteine, PBS (pH 6, pH 7.4 and pH 8) solution, respectively and incubated for 30 min. The stability and the identity of gold nanoparticle were measured by recording UV absorbance after 30 min. The biomedically important plant mediated gold nanoparticles were autoclaved and it was analysed by UV–vis spectrophotometer.

Results and discussion

Synthesis of gold nanoparticles

Formation of gold nanoparticles by reduction of aqueous metal ions during exposure of microwave radiation to the plant extract may be easily followed by UV–vis spectroscopy. It is well known that the gold nanoparticles exhibit ruby red colour (Figure 1) in water. The appearance of ruby red colour is the characteristics of gold nanoparticles it clearly indicates formation of gold nanoparticles. This colour formation is due to the surface Plasmon vibration of the metal nanoparticles [20]. In case of gold nanoparticles, the narrow surface Plasmon resonance band occurred at 520 nm as shown in figures.

Figure 1
figure 1

Visual observations (a) Plant leaf extract (b) gold chloride solution and (c) formation of gold nanoparticles.

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Reduction of gold ions in to gold nanoparticles is the time taking process, in early studies on synthesis of gold nanoparticles using microorganism required 2 to 120 hrs [21–23, 6], but our plant extract stabilized microwave radiation technique studies shows rapid formation of gold nanoparticles within a minute. Using of microwave radiation to nanoparticles synthesis has benefit that it provides uniform heating of aqueous solution and prevent the particles from aggregation [24].

Production of optimized gold nanoparticles

The process of optimization of gold nanoparticles was carried out by keeping the gold concentration and volume of plant extract solution as constant and changing the microwave radiation power time and plant extract concentration. Figures 2, 3, 4, 5 and 6 represents UV-visible absorption spectra of Hibiscus rosa-sinensis stabilized gold nanoparticle formed when changing the process variables like concentration of plants extract from lower to higher concentration (2–10%), microwave radiation power from 140–420 W and time of exposure from 30–90 sec. From the absorption spectra it can be seen that plant extract concentration plays an important role in controlling the size and shape of nanoparticles. A single and narrow absorbance band was observed at 520 nm which is characteristics for the formation of small nanoparticles which was confirmed by TEM result. While increasing the leaf extract concentration up to 8%, the absorbance intensity steadily increased as a function of time of reaction. At 10% leaf extract concentration treated with gold ion solution, the absorbance decreased due to the bioavailability of functional groups in leaf extract for gold ions leads to the competition between leaf extract and metal ions for reduction process (Figure 6).

Figure 2
figure 2

2% of Plant extracts and microwave heating at three different temperature.

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Figure 3
figure 3

4% of Plant extracts and microwave heating at three different temperatures.

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Figure 4
figure 4

6% of Plant extracts and microwave heating at three different temperature.

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Figure 5
figure 5

8% of Plant extracts and microwave heating at three different temperature.

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Figure 6
figure 6

10% of Plant extracts and microwave heating at three different temperature.

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Among the variables, plant extract 8% concentration microwave radiation power 420 W and microwave exposure time in 90 sec, was ideal for gold nanoparticles preparation, because it shows (Figure 5) gold surface resonance occur at ~520 nm and steadily increased in intensity as a function microwave power and time of reaction without any shift in peak wavelength. There is no change in peak wavelength suggest that particles are monodispersed in the aqueous solution without aggregation [4].

Stability testing

The stability of Hibiscus rosa-sinensis stabilized gold nanoparticles was evaluated by monitoring the plasmon λmax in 0.2 M cysteine, 10% NaCl, phosphate buffer solution at pH 6, 7.4 and 8. The plasmon wavelength in all above shows shift of ~1 – 8 nm. Our result from the in-vitro stability studies has confirmed that gold nanoparticles were stable in biological fluids at physiological pH (Figure 7).

Figure 7
figure 7

Stability analysis of gold nanoparticles (a) by various parameters and (b) absorbance before and after autoclaving.

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Gold nanoparticles used varies biomedical application before going to in-vivo models. Sterilization is very important process to complete destruction of all organism including bacteria, spores, virus.

Several sterilization methods can be used, including physical methods such as autoclaving and UV irradiation, which comprise moist heat and drug heat, respectively and chemical treatment such as using hydrogen peroxide gas plasma, ethylene oxide and chemical vapour, which include both gaseous and liquid solutions.

Sterilization remains a critical step for the in-vivo use of gold nanoparticles, and the effects of sterilization on the integrity of the physiochemical properties of gold nanoparticles used to be investigated. Hence, gold nanoparticles sterilized by autoclave at 120°C for 15 min before and after sterilization of gold nanoparticles shows similar surface plasmon resonance peak ~520 nm. It indicates that the gold nanoparticles size did not changed by sterilization. The particles are thus stabilized by the capping agent like flavonoids and water soluble compounds present in the leaf extract [25].

TEM

A TEM study reveals the size and shape of nanoparticles. The shape of gold nanoparticles prepared in this study is spherical with size in the range of 16 – 30 nm (Figure 8). Some nanoparticles are seen as aggregated. In the surface of the nanoparticles some light colour of materials found that may be the stabilizing agent responsible for the nanoparticles synthesis [26]. The separation between nanoparticles was observed from TEM image due to the presence of capping agent which is characteristics to well dispersed nanoparticles formation in the optimized conditions.

Figure 8
figure 8

TEM image of gold nanoparticle synthesized from Hibiscus rosa-sinensis.

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FT-IR

The plants are having a lot of bio-chemical molecules like stigmasterol, erogosterol, Hibiscentin and falvonoids play an important role in synthesis and stabilizing of gold nanoparticles [27, 28]. These compounds may actively involve in the reduction of gold ions to gold nanoparticls was characterized by FTIR spectrum of leaf extract before and after reduction process. In the FTIR spectrum (Figure 9) of Hibiscus rosa-sinensis multiple peaks were observed between 735 and 1059 cmˉ1 representing various functional groups of the alkaloids and flavonoids and other bimolecular functions. There were no amides groups present in the synthesized gold nanoparticles powder. After the bioreduction of HAuCl4 by the extract of Hibiscus rosa-sinensis considerable shifts were observed in the IR spectrum of Hibiscus rosa-sinensis gold nanoparticle. Major shifts observed were the peak at 1611 cm−1 shifted to 1656 cm−1 and the peak at 1383 cm−1 disappeared. The medium and sharp peak at 1059 shifted to 1083 cm−1 and changed into small blunt peak. The peaks observed show that alkaloids or flavonoids would have adsorbed on the metal surface by the interaction with carbonyl group/keto groups. Similarly the functional groups in the Hibiscus extract could have been oxidized during the reduction of HAuCl4 to gold nanoparticles. The shifted peaks in the plants extracts and nanoparticles clearly indicates the nanoparticles formation by the plant extracts of Hibiscus rosa-sinensis acts as reducing and stabilizing agent [29, 30].

Figure 9
figure 9

FT-IR spectrum of (a) Hibiscus rosa-sinensis leaf extract and (b) gold nanoparticles.

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Conclusions

By using green chemistry, the gold nanoparticles were synthesized by microwave radiation method which is a rapid method and gives excellent reproducibility when compared to other synthetic processes. Using plants for nanoparticle synthesis can be advantageous over other biological process by eliminating the elaborate process of maintaining cell cultures. The gold nanoparticles have showing high stability rate in ‘in-vitro’ conditions and can be applicable for biological applications. Thus the field of gold nanoparticle biosynthesis is leading to avenues in material science, chemistry, nanobiotechnology and biotechnology. The single-step green process has shown to be effective for the generation and stabilization of non-toxic gold nanoparticles for different applications in a myriad of diagnostic purpose and therapeutic applications. Hibiscus rosa-sinensis mediated eco-friendly synthesized gold nanoparticles have excellent stability under different chemicals treatment and sterilization. Due to this stability characterization of gold nanoparticles could be used for biomedical and sensor applications. Thus the design and development of ‘green’ gold nanoparticles can be safely produced, stored and shipped worldwide.

References

  1. Rajeshkumar S, Kannan C, Annadurai G: Synthesis and characterization of antimicrobial silver nanoparticles using marine brown seaweed Padina tetrastromatica . Drug Invent. Today 2012,4(10):511–513.

    Google Scholar 

  2. Malarkodi C, Rajeshkumar S, Paulkumar K, Gnanajobitha G, Vanaja M, Annadurai G: Eco-friendly synthesis and characterization of gold nanoparticles using Klebsiella pneumonia . J. Nanostruct. Chem. 2013,3(30):1–7.

    Google Scholar 

  3. Vanaja M, Gnanajobitha G, Paulkumar K, Rajeshkumar S, Malarkodi C, Annadurai G: Phytosynthesis of silver nanoparticles by Cissus quadrangularis - influence of physico-chemical factors. J. Nanostruct. Chem. 2013,3(17):1–8.

    Google Scholar 

  4. Rajeshkumar S, Malarkodi C, Vanaja M, Gnanajobitha G, Paulkumar K, Kannan C, Annadurai G: Antibacterial activity of algae mediated synthesis of gold nanoparticles from Turbinaria conoides . Der. Pharma. Chemica. 2013,5(2):224–229.

    Google Scholar 

  5. Gnanajobitha G, Paulkumar K, Vanaja M, Rajeshkumar S, Malarkodi C, Annadurai G, Kannan C: Fruit mediated synthesis of silver nanoparticles using Vitis vinifera and evaluation of their antimicrobial efficacy. J. Nanostruct. Chem. 2013,3(67):1–6.

    Google Scholar 

  6. Malarkodi C, Rajeshkumar S, Paulkumar K, Vanaja M, GnanaJobitha G, Annadurai G: Bactericidal activity of bio mediated silver nanoparticles synthesized by Serratia nematodiphila . Drug Invent. Today 2013,5(3):1–7.

    Google Scholar 

  7. Vanaja M, Rajeshkumar S, Gnanajobitha G, Paulkumar K, Malarkodi C, Annadurai G: Kinetic study on green synthesis of silver nanoparticles using Coleus aromaticus leaf extract. Adv. Appl. Sci. Res. 2013,4(3):50–55.

    Google Scholar 

  8. Gnanajobitha G, Rajeshkumar S, Kannan C, Annadurai G: Preparation and characterization of fruit-mediated silver nanoparticles using pomegranate extract and assessment of its antimicrobial activity. J. Environ. Nanotechnol. 2013,2(1):04–10.

    Google Scholar 

  9. Vanaja M, Annadurai G: Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Appl. Nanosci. 2012, 3: 217–223.

    Article  Google Scholar 

  10. Karthiga P, Soranam R, Annadurai G: Alpha-mangostin, the major compound from Garcinia mangostana Linn. Responsible for synthesis of Ag nanoparticles: its characterization and evaluation studies. Res. J. Nanosci. Nanotechnol. 2012,2(2):46–57.

    Article  Google Scholar 

  11. Wang Y, He X, Wang K, Zhang X, Tan W: Barbated skullcup herb extract-mediated biosynthesis of gold nanoparticles and its primary application in electrochemistry. Colloids Surf. B: Biointerfaces 2009,73(1):75–79.

    Article  Google Scholar 

  12. Song JY, Jang HK, Kim BS: Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Process Biochem. 2009,44(10):1133–1138.

    Article  Google Scholar 

  13. Noruzi M, Zare D, Khoshnevisan K, Davoodi D: Rapid green synthesis of gold nanoparticles using Rosa hybrid petal extract at room temperature. Spectrochim. Acta A 2011,79(5):1461–1465.

    Article  Google Scholar 

  14. Das RK, Gogoi N, Bora U: Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract. Bioproc. Biosyst. Eng. 2011,34(5):615–619.

    Article  Google Scholar 

  15. Lin L, Wang W, Huang J, Li Q, Sun D, Yang X, Wang H, He N, Wang Y: Nature factory of silver nanowires: plant mediated synthesis using broth of Cassia fistula leaf. Chem. Eng. J. 2010,162(2):852–858.

    Article  Google Scholar 

  16. Cruz D, Fale PL, Mourato A, Vaz PD, Luisa Serralheiro M, Lino ARL: Preparation and physicochemical characterization of Ag nanoparticles biosynthesized by Lippia citriodora (Lemon Verbena), colloid. Surf. B Interf. 2010,81(1):67–73.

    Article  Google Scholar 

  17. Olagbende-Dada SO, Ezeobika FN, Duru FI: Anabolic effect of Hibiscus rosa-sinensis Linn. Leaf extracts in immature albino male rats. Nig. Q. J. Hosp. Med 2007,17(1):5–7.

    Article  Google Scholar 

  18. Sharma S, Sultana S: Effect of Hibiscus rosa-sinensis extract on hyperproliferation and oxidative damage caused by benzoyl peroxide and ultraviolet radiations in mouse skin. Basic Clin. Pharmacol. Toxicol. 2004,95(5):220–225.

    Article  Google Scholar 

  19. Sachdewa A, Khemani LD: A preliminary investigation of the possible hypoglycemic activity of Hibiscus rosa-sinensis . Biomed. Environ. Sci. 1999,12(3):222–226.

    Google Scholar 

  20. Rajeshkumar S, Malarkodi C, Paulkumar K, Vanaja M, Gnanajobitha G, Kannan C, Annadurai G: Seaweed mediated synthesis of gold nanoparticles using Turbinaria conoides and its characterization. J. Nanostruct. Chem. 2013,3(44):1–7.

    Google Scholar 

  21. Rajeshkumar S, Kannan C, Annadurai G: Green synthesis of silver nanoparticles using marine brown algae Turbinaria conoides and its antibacterial activity. Int. J. Pharm. Bio. Sci. 2012,3(4):502–510.

    Google Scholar 

  22. Husseiny MI, Abd El-Aziz M, Badr Y, Mahmoud MA: Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa . Spectrochim. Acta A 2007, 67: 1003–1006.

    Article  Google Scholar 

  23. Mukherjee P, Senapati S, Mandal D, Ahmad A, Khan MI, Kumar R, Sastry M: Extracellular synthesis of gold nanoparticles by the fungus Fusarium oxysporum . Chembiochem 2002, 3: 461–463.

    Article  Google Scholar 

  24. Saifuddin N, Wong CW, Yasumira AAN: Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. E-J. Chem. 2009,6(1):61–70.

    Article  Google Scholar 

  25. Philip D, Unni C, Aromal SA, Vidhu VK: Murraya Koenigii leaf-assisted rapid green synthesis of silver and gold nanoparticles. Spectrochim. Acta A 2011, 78: 899–904.

    Article  Google Scholar 

  26. Iosin M, Baldeck P, Astilean S: Study of tryptophan assisted synthesis of gold nanoparticles by combining UV–Vis, fluorescence, and SERS spectroscopy. J. Nanopart. Res. 2010, 12: 2843–2849.

    Article  Google Scholar 

  27. Philip D, Unni C: Extracellular biosynthesis of gold and silver nanoparticles using Krishna tulsi ( Ocimum sanctum ) leaf. Phys. E. 2011,43(7):1318–1322.

    Article  Google Scholar 

  28. Mohammed-Fayaz A, Girilal M, Venkatesan R, Kalaichelvan PT: Biosynthesis of anisotropic gold nanoparticles using Maduca longifolia extract and their potential in infrared absorption. Colloid. Surf B: Biointerf. 2011,88(1):287–291.

    Article  Google Scholar 

  29. Philip D: Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract. Spectrochim. Acta A 2009, 73: 374–381.

    Article  Google Scholar 

  30. Raghunandan D, Basavaraja S, Mahesh B, Balaji S, Manjunath SY, Venkataraman A: Biosynthesis of stable polyshaped gold nanoparticles from microwave-exposed aqueous extracellular anti-malignant guava ( Psidium guajava ) leaf extract. Nano Biotechnol. 2009,5(1–4):34–41.

    Google Scholar 

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Acknowledgement

Authors gratefully acknowledge to Dr. T.P. Sastry, Scientist, CLRI for providing lab and characterization facilities for do the research.

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Authors and Affiliations

  1. Department of Biochemistry, Adhiparasakthi College of Arts and Science, Kalavai, Vellore District, 632506, TN, India

    Akbar Yasmin, Kumaraswamy Ramesh & Shanmugam Rajeshkumar

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  1. Akbar Yasmin
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  2. Kumaraswamy Ramesh
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  3. Shanmugam Rajeshkumar
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Correspondence to Shanmugam Rajeshkumar.

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The author(s) declare that they have no competing interests.

Authors’ contributions

AY and KR completed the nanoparticles synthesis and Characterization and SR carried out the manuscript preparation. All authors read and approved the final manuscript.

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Yasmin, A., Ramesh, K. & Rajeshkumar, S. Optimization and stabilization of gold nanoparticles by using herbal plant extract with microwave heating. Nano Convergence 1, 12 (2014). https://doi.org/10.1186/s40580-014-0012-8

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  • DOI: https://doi.org/10.1186/s40580-014-0012-8

Keywords

  • Gold nanoparticles
  • Green synthesis
  • Microwave
  • Stability
  • Optimization